- A specific footprint of cellular events
- Isothermal Microcalorimetry (IMC)
- An open platform that changes scientific development
Isothermal Microcalorimetry (IMC) is specific in that its samples are kept at a constant temperature during the course of the experiment. IMC measures the total metabolic status and response of a cellular system, thereby giving the potential for rapid identification and quantification. This makes IMC a true phenotype assay with great benefit in that very little Information is required about the system to be studied. Because there is no need to know the pathways or receptors involved in a process, novel systems can be studied directly, leading to faster results in drug discovery and cell research. This can potentially be used for rapid identification and quantification.
- Possibility of 3-D: A holistic approach and a new dawn for drug discovery
- IMC is a label-free technology, continuously provides important time-resolved data, is non-destructive, has few limits in morphology other than size, is sensitive and fast, is low cost to run, and is nonspecific.
The CalScreener® Principle
Biological processes caused by physical, chemical or biological stimuli in which metabolic changes are anticipated are all valid for the analysis.
The calPlate containing the individual sealed cups holding the cell culture are placed in a thermostatic chamber set at the target temperature with a precision within thousands of a Kelvin.
The cups rest upon a heat-flux detecting sensor, the thermopile. The sensor is attached to a heat-sink with a large mass compared to the cell-culture cups. All heat produced is transferred to the heat-sink giving rise to a signal in the thermopile sensor proportional to the heat-flow.
The measured heat is thus independent of the model system or the process involved. We have a label free, real-time, detection system applicable to a wide range of biological applications.